Mini-PROTEAN® TGXStain-Free™ 预制胶不仅继承了TGX预制胶快速稳定、保存时间长的优点,而且在电泳后无需染色,可立刻使用Stain Free™ 成像系统(GelDoc™ EZ或ChemiDoc™ MP)对蛋白质进行荧光检测。TGX免染胶具有类似Laemmli系统的分离特性,兼容传统的Tris/Glycine缓冲系统,可在15分钟内完成电泳,并且在2.5分钟内实现显色成像。TGX免染胶基质内含有特殊的三卤化合物,该化合物在特定紫外光激发下可与蛋白质结合,并发出荧光,通过GelDoc™ EZ或ChemiDoc™ MP成像系统可在凝胶上或印迹膜上轻松检测成像。
优势特点
·电泳时间只需15分钟
·转印速度非常快,可在印迹膜上验证转印效率
·使用Laemmli缓冲液系统,运行成本低
·电泳后无需染色,5分钟内可显色成像
·与考马斯染料的灵敏度相当
·与染色法相比,具有更高的重复性和定量准确性
·可在一块凝胶上同时实现显色成像、转膜和质谱分析
订购信息
4568023:7.5%Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
4568033:10%Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
4568043:12%Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
4568083:4–15%Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
4568093:4–20%Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
4568103:8–16%Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
4568123:AnykD™ Mini-PROTEAN® TGXStain-Free™ ProteinGels,10well,30ul
技术参数
Gel dimensions (W x L x thickness), cm | 8.6 x 6.7 x 0.1 |
Cassette dimensions (W x L x thickness), cm | 10 x 8 x 0.46 |
Cassette material | Styrene copolymer |
Comb material | Polycarbonate |
Gel storage conditions | Store flat at 2–8°C; do not freeze |
Shelf life at recommended temperature* | 12 months |
Recommended sample buffer (Laemmli, dilute 1:1 with sample) | 62.5 mM Tris-HCI pH 6.8. 2% SDS, 25% glycerol, 0.01% bromophenol blue |
Recommended running buffer (Tris/Glycine/SDS) | 25 mM Tris-HCI, 192 mM glycine, 0.1% SDS, pH 8.3 |
Gel chemistry | TGX |
Gel format | Mini |
Opening lever | 100% aluminum and recyclable |
* From date of manufacture. | |