产品优势:
(1)无血清、无需滋养层细胞;可收集无外源干扰的纯净外泌体;
(2)可应用于iPSC工业化2D和3D大规模培养和扩增;
(3)自主化、国产化的原研体系;供货稳定,高性价比;
(4)支持临床级/药品级细胞培养,培养的iPSC细胞通过了中国食品药品检定研究院质量复核;iPSC来源功能药物通过了IND默示许可。
产品规格
产品 | 规格 | 货号 | 存储条件 |
hiPSC/hESC培养基-ncEpic* | 1Kit | RP01001 | - |
ncEpic Basal Medium | 496mL | RP01001-01 | 2℃~8℃ |
ncEpic 125x Supplement | 4 mL | RP01001-02 | -20℃~-80℃ |
将基础液和添加物混匀配置成完全培养基,可在2℃~8℃中存储,2周内用完。
细胞形态及功能鉴定
A、B、C:连续培养的hiPSC细胞株细胞形态图示。标尺:200μm。
D:细胞表面标志物检测:SSEA4+,Tra-1-81+。E:细胞多能基因检测:OCT4+,NANOG+。
F:连续培养的hiPSC(P30)细胞核型检测:46,XX。
文献引用
Base editing-mediated splicing correction therapy for spinal muscular atrophy
2020. Xiang Lin, et al. Cell Research(IF 46.297).
Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice
2019.Jinjing Li,et al.National Science Review(IF 23.178).
Inducible Pluripotent Stem Cel-Derived Sma Extracelular Vesicles Reuvenate Senescent lood-Brain Barrier to Protect against ischemic Stroke in Aged .
2022. Qing Li, et al. ACS Nano(IF 18.0280).
ESC-sEVs Rejuvenate Senescent Hippocampal NSCs by Activating Lysosomes to Improve Cognitive Dysfunction in Vascular Dementia
2020. Guowen Hu, et al. Advanced Science(IF 17.521).
Human ESC-sEVs alleviate age-related bone loss by rejuvenating senescent bone marrow-derived mesenchymal stem cells
2020. Liangzhi Gong, et al. Journal of Extracellular Vesicles(IF 17.3378).
ESC-SEVs Reiuvenate Aging Hippocampal NSCs by Transferring SMADs to Regulate the MYT1-Eqln3-Sirt1 Axis
2021. Guowen Hu. Molecular Therapy (IF 12.9101)
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